CANsure

CANsure

Air quality of laboratory is very crucial for success of ART procedure.VOC (volatile organic compound) is one of the factors that affects air quality of laboratory. There are various sources of VOCs like perfume, after shave, paint, Iso propyl alcohol, aerosol, organic solvents, fixatives, certain disinfectants, outdoor fumes from motor vehicles or industrial gas etc. The maximum care should be taken to avoid VOCs in the laboratory to get good outcome of ART procedure. Use of VOC free cleaning agent is a one step towards the improving quality of air.

 

CANsure is VOC free cleaning solution. It is sperm survival tested cleaning solution which assures efficient application in the laboratories. Follows are the advantages of the product

  • VOC free
  • Non toxic
  • Odourless
  • Non corrosive
  • Non staining and non tainting
  • Easy to use, single step cleaning
  • Suitable for all surfaces like metal, glass, plastic etc.
  • Good for cleaning of laboratory floor and wall

Available as:

  • CANsure for CO2 Incubator and Laminar Flow       

Used in laboratories for cleaning incubators, flow hoods and other instruments.

  • CANsure for Floor  

Used in laboratories for cleaning Floor and walls.

  • CANsure Hand Cleaning Solution

Used to clean hands prior to and post of any process in laboratory

Range of product & Packing Size

CANsure for Incubator

CANsure for Incubator

CANsure for Hands

FAQs

If the sperm count in the semen sample is more, than dilute the sample with Phosphate Buffer Saline (PBS) or sperm preparation media to the concentration about 15-20 million per ml. And if the count is less, then centrifuge the semen sample at 800-1000 rpm for 10 minutes and then proceed for slide preparation.

The kit is applicable to fresh, raw, diluted, frozen/thawed and processed semen sample.

To maintain 90°C for melting of gel one can use boiling water bath or dry bath and to maintain 22°C cooling plates.

After heating the gel tube at 90°C, do not allow the gel temperature to go below 37°C, as gel has the property to get solidify when it gets cool down after digestion. Proceed for slide preparation immediately, when the vial temperature reaches nearly 37°C.

Uneven gel distribution and bubble formation on the slide while placing the coverslip may disturb the integrity of the gel.
After placing sample over the slide maintain proper temperature and time to get the gel solidify properly.
Jerky overlay of the solution might affect the integrity of the gel so gently overlay the solution.

Always prepare fresh stain before proceeding for slide staining. If the staining is too dark then destain the slide completely with 100% ethanol, air dry and again stain by decreasing the time for staining but maintaining rest of the conditions same. If the staining is light then destain the slide with 100% ethanol and then restain by increasing the time for staining.

Avoid adding of semen sample when the gel in Agarose Gel Tube is too hot as high temperature affects DNA of the sperms. Also avoid vigorous mixing of the semen sample and bubble formation in gel as it generates stress which ultimately affects the integrity of DNA.

DFI (DNA Fragmentation Index) is the ratio expressed as percentage of sperm having fragmented DNA to the total sperm count.DNA fragmentation in sperm may be a cause of male infertility which cannot be detected by routine semen parameters like sperm concentration, motility analysis and morphology assessment.

Following equation can be used to calculate SDF value:
SDF Value (%) = (Number of fragmented Sperms scored / Total number of Sperms scored) X 100

SDF Reference Value : 25%. The values obtained after SDF calculation must correspond with all the earlier clinical and laboratory findings of the respective semen sample. The test offers presumable quantitative information of DNA Fragmentation of sperm.

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