CANfrag - DNA fragmentation test kit for sperm

CANfrag - DNA fragmentation test kit for sperm

CANfrag DNA fragmentation Test kit is designed to detect DNA fragmentation in human sperm. The valuable and intellectual afford of our team made the kit, easy to perform and score. The results obtained with CANfrag are consistent and reliable. This is SCD test based kit. The working principle of the kit based ondenaturation of DNA to assess and differentiate between fragmented and intact DNA in spermatozoa. Untreated spermatozoa are embedded between two layers of base agarose and inert top micro gel, which are then treated with acid to generate restricted single stranded DNA motifs from DNA breaks.

 

Following this, sequential lysis treatment is provided for removal of nuclear proteins. Staining with dye makes the halo and core visible under bright field microscope. The sperm with minimal DNA fragmentation or without breaks forms large halos of intact DNA loops around the central core. The sperm with fragmented DNA forms either very minimum sized halo or no halo formation. DNA fragmentation in sperm may be a cause of male infertility which cannot be detected by routine semen parameters like sperm concentration, motility analysis and morphology assessment.

Kit Contents

The steps performed for procedures are briefly describe in the tutorial and the leaflet provided with the product.

Some Causes Of DNA Fragmentation Of Sperm

  • High Level of ROS
  • Advanced Age
  • Elevated Scrotal Temperature
  • Varicocele
  • Febrile Illness
  • Gonadotoxins( e.g. cancer therapy)
  • Obesity
  • Smoking/ drug addiction

The Consequences of DNA Fragmentation of human sperm

  • Infertility
  • Multiple failure of IVF/ICSI
  • Poor blastocyst development
  • Repeated miscarriage in partner

Impact of DNA fragmentation analysis in human sperm

  • Help clinician for management and treatment of male Infertility
  • Life style improvement

Feature of CANfrag- DNA fragmentation Determination kit for human sperm

  • Short process time is approx. 35minutes
  • Large testing area on slide which gives
  • Reliable distribution of Sperm cells
  • Avoid overlapping of cells
  • Larger area for scoring
  • Enhance the accuracy of test
  • No special instruments require
  • Reliable and reproducible result

Free Reporting software

  • To generate report
  • To maintain historical data of last five reports of the same patient.
  • The main user can export the data to the excel sheet to maintain the data.

Packing Size

05

A kit of 5 tests

10

A kit of 10 tests

FAQs

If the sperm count in the semen sample is more, than dilute the sample with Phosphate Buffer Saline (PBS) or sperm preparation media to the concentration about 15-20 million per ml. And if the count is less, then centrifuge the semen sample at 800-1000 rpm for 10 minutes and then proceed for slide preparation.

The kit is applicable to fresh, raw, diluted, frozen/thawed and processed semen sample.

To maintain 90°C for melting of gel one can use boiling water bath or dry bath and to maintain 22°C cooling plates.

After heating the gel tube at 90°C, do not allow the gel temperature to go below 37°C, as gel has the property to get solidify when it gets cool down after digestion. Proceed for slide preparation immediately, when the vial temperature reaches nearly 37°C.

Uneven gel distribution and bubble formation on the slide while placing the coverslip may disturb the integrity of the gel.
After placing sample over the slide maintain proper temperature and time to get the gel solidify properly.
Jerky overlay of the solution might affect the integrity of the gel so gently overlay the solution.

Always prepare fresh stain before proceeding for slide staining. If the staining is too dark then destain the slide completely with 100% ethanol, air dry and again stain by decreasing the time for staining but maintaining rest of the conditions same. If the staining is light then destain the slide with 100% ethanol and then restain by increasing the time for staining.

Avoid adding of semen sample when the gel in Agarose Gel Tube is too hot as high temperature affects DNA of the sperms. Also avoid vigorous mixing of the semen sample and bubble formation in gel as it generates stress which ultimately affects the integrity of DNA.

DFI (DNA Fragmentation Index) is the ratio expressed as percentage of sperm having fragmented DNA to the total sperm count.DNA fragmentation in sperm may be a cause of male infertility which cannot be detected by routine semen parameters like sperm concentration, motility analysis and morphology assessment.

Following equation can be used to calculate SDF value:
SDF Value (%) = (Number of fragmented Sperms scored / Total number of Sperms scored) X 100

SDF Reference Value : 25%. The values obtained after SDF calculation must correspond with all the earlier clinical and laboratory findings of the respective semen sample. The test offers presumable quantitative information of DNA Fragmentation of sperm.

Looking for more details?

Become A Distributor

Fill up simple form to initiate the procedure.