A kit to Sort Sperm Using Magnetic Separator
Apoptosis is the programmed cell death and involves series of morphological and biochemical changes that eventually results in cellular death. The extrusion of phosphotidyl serine (PS), which in normally present on the inner leaflet of plasma membrane, to the outer surface has been identified as one of the early apoptotic changes in human spermatozoa.
This is a non-invasive method to select non-apoptotic sperm using magnetic separation technique. The paramagnetic nanobeads are conjugated with Annexin V, a 35-36 kDa phospholipid binding protein that has selective affinity to bind with externalized phosphotidyl serine. The magnet in the system retains the apoptotic sperm labeled with microbeads and the unlabeled spermatozoa can be used for IUI/IVF/ICSI.
The kit can be used for fresh, washed and frozen-thaw sample. The components of the kit are free from human and animal derived ingredients.
Better Cryo-survival rate of sperm
The steps performed for procedures are briefly describe in the tutorial and the leaflet provided with the product.
It is advised to have double density wash method to remove debris that may cause interference while sperm separation.
Up to 50 million of the sperm count can be taken for performing single assay.
If the concentration of sperm is higher than 50 million, the volume of the reagents will increase proportionately. Means if the sperm concentration is 75 million then take the reagents 1.5 times more than as define.
If the tube is removed from the magnet assembly then the apoptotic sperm will no longer be in contact with the magnetic field and may get mix with the non-apoptotic sperms. So it is emphasized to keep the tube in magnet assembly only while aspirating the non-apoptotic sperms.
The sperms that does not express apoptotic marker will not get separated during the procedure. So if the non-motile sperms are not expressing the apoptotic marker they will be present in the aspirated suspension.
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