CANezy offers simple, easy and safe method to separate spermatozoa based on motility of Sperm for ART (Assisted Reproductive Technology) procedures. The device minimizes mechanical stress and reactive oxygen species (ROS) generation associated with conventional centrifugation methods.
The result is a cleaner, functionally competent sperm population suitable for use in assisted reproductive techniques.
Variable volume in single device (0.5-3.0 ml)
Close device- Reduces evaporation of the sperm preparation medium
No risk of spillage and contamination
CANezy- Sperm separation device is a close system integrated with microporous filter membrane that allows the sperm to swim through the filter and the separated sperms are then used for ART procedures.
No. The required incubation time depends on the concentration of progressively motile sperm in the semen sample. Samples with higher motility generally require a shorter incubation period.
A minimum sperm concentration of 2 million/mL is required; there is no specified upper limit.
CANezy is the device that allows adjustment of the semen sample volume from 0.5 mL to 3 mL, while maintaining a fixed 0.7 mL volume of separation medium.
Fresh (raw) or frozen–thawed sample.
Dilute the sample with media, mix gently and use for the separation. It is recommended to check the sample for progressive motile sperms.
Elevated viscosity may compromise efficient sperm separation. Dilute the sample with media and try to reduce the viscosity without excessive manipulation.
If the incubation period exceeds, then there are changes of getting sluggish motile sperms and non-motile sperm.
When there is a delay in using the separated sperm, remove the separated. sperm from the device rather than incubating for longer time and store in appropriate sterile tube.
If incubating in HEPES and/or MOPS buffered media then incubate in non-gassed incubator and if using Bicarbonate buffered media use CO2 incubator.
There is a possibility that sperms having round head and small acrosome, yet retaining good motility, may pass through the filter membrane during the incubation period.
CANezy is simple to operate and requires no prior training or specialized skills. The only prerequisite is maintaining aseptic conditions throughout the procedure.
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